The Use of the Green Fluorescent Protein and Its Application in the Study of Biochemistry

Green Fluorescent ProteinApplications in Biochemistry, Cell Biology and BiotechnologyThe unripe clarification protein (GFP) of Aequorea victoria is a unique in vivo receptor for supervise dynamic processes in cubicles or organisms (Gerdes & amp; Kaether 1996, p.44). This Union Atlantic organism is highly organized in structure, with an abominable great power to componentrate and emit green fluorescent light from photocytes at the margin of its umbrella, fashioning it a perfect dumbfound for cellular studies. The molecular cloning of GFP and its demonstration by Chalfie (1988) has opened up exciting new avenues of investigation in cell biology. The broad flexibility as a non-invasive stigma in quick cells allows for numerous applications of GFP (Yang F. et al. 1996, p.1246) such as a reporter for gene expression, as well as a marker to strike cell lineage during development. Thus, its ability to function as a protein tag allows us to localise proteins in victuals cel ls, as it tolerates N- or C- terminal fusions to a broad variety of proteins (Tsien R.S. 1998, p.511). These applications of GFP ar now found to be more weapons-grade to naturalized methods in cell biology. GFP is a 238 amino back breaker protein with an unembellished molecular weight of about 27-30 kDa on SDS-PAGE (Gerdes & Kaether 1996, p.44).

The precious cylindrical fold of the protein seems ideally suited for its function, where cardinal promoters fellow to purposeher to form a dimer in the crystal, making it fitting to undergo energy transfer (Yang F. et al. 1996, p.1248). Eleven anti-parallel important strands use up up the densely compact beta-barrel, where sha! rp sections of the alpha whorl form internal caps on the cylinders (Prasher D.C. et al. 1992, p. 229). This irregular alpha spiraling segment provides a scaffold for the chromophore which is laid in the nonrepresentational centre of the cylinder. The structure is... If you want to get a luxuriant essay, order it on our website: BestEssayCheap.com

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